Scheduling

• On-line scheduling calendar
• Self-operation available only to trained personnel. Calendar link provided on completion of training.

BD FACSAria

• Call or e-mail Nan Sheng
• nas260@pitt.edu
• 412-624-4434
• View current schedule.
• Schedule all time points for an experiment prior to starting the experiment to ensure availability of instrument and operator.
• DO NOT schedule an appointment until all reagents are in-house or a confirmed shipping date has been received.
• Minimum booking time is 30min.
• Facility must be notified via email of all cancellations by noon 2business days prior to appointment to avoid charges.
• Facility must authorize significant changes to an appointment. Significant changes are defined as a greater than 20% increase/decrease of tube numbers, changing between analysis and sorting, a greater than 10% increase/decrease of total cells/sort tube. Unauthorized work will not be permitted to impinge on other reserved time.
• Scheduling: ALL appointment requests MUST provide following upon request. This will be strictly enforced.
• Valid account number on file
• PI/Grant Name
• Your name and contact info
• Species, Cell Type and approximate cell size (in microns)
• Known biohazards
• All colors used in experiment
• Analysis: number of tubes, not including compensation tubes, and preferred event number to acquire
• Sort: number of tubes to be sorted, approximate total cell number per tube and collection device (tube, slide, plate)
• Preferred dates/times
• Day of Appointment:
• A confirmed appointment and completed requisition form
• Appropriate compensation tubes: negative and single color positive control tubes
• Filtered, clump-free samples in phenol red-free buffer
• Sort cell concentration: 5-10e6 cells/ml
• Minimum cell number and volume:
• Analysis: 2e5 cells/tube, 200µl/tube in microfuge tubes
• Sorting: less than 1e6 bulk cells in 300µl in microfuge tubes
• Appropriate collection media (phenol red-free) plus serum, 10-20ml